A METHOD FOR PREPARING OF FUSICOCCIN PRODUCENT FUSICOCCUM AMYGDALI

摘要

This invention pertains to the microbiological industry, namely methods of obtaining biologically active substances, and, to be more specific, the method of obtaining the seed grain of the mycelia fungi Fusicoccum amygdali Del. for the biosynthesis of the vegetable growth regulator fusicoccin. The purpose of this invention is to develop the method of obtaining the seed grain of the standardized fungi F. amygdali Del. by growing the producer in flasks and in the fermentor with the purpose of intensifying the process of fusicoccin synthesis and increasing the output of fusicoccin. This purpose is achieved by using a more effective and cheaper nutrient medium for seed grain to obtain the fungi F. amygdali Del. seed grain. The proliferation of seed grain is carried out in 2 stages - in flasks and in the fermentor. The optimum amount of seed grain should be used for inoculation in the fermentors, and the disintegration of the producer conglomerates should be carried out too. The technical effect is achieved by growing the seed grain in two stages - in flasks and in the seeding fermentor in the following nutrient medium (g/l): crude sugar - 20-24; soy flour - 13-15; twice-replaced potassium phosphate - 2.8-3.2; potassium sulphate - 0.2-0.3; magnesium sulphate - 0.2-0.3; water - up to 1 liter. To inoculate fermentors, 8-10% of the seed grain is used. Cultivation in the seeding fermentor is carried out for 21-24 hours, the mycelia disintegration is carried out directly in the apparatus before growing in the seeding fermentor and in the production fermentor.The suggested method allows the intensification of the periodic process of obtaining fusicoccin, and ensures the accumulation of fusicoccin at the concentration of 600-800 mg/l in 54-66 hours of fermentation.