Method of assessing the degree of protein denaturation in biological tissues, based on the tomographic image obtained by the magnetic nuclear resonance.

摘要

Method of assessing the degree of protein denaturation in biological tissues is based on the tomographic image obtained by the magnetic nuclear resonance. Changes of tissue properties are monitored through tomographic visualisation by the magnetic nuclear resonance method. In particular, the speed of the processes of spin-lattice and spin-spin relaxation are monitored. The method also involves the implementation of long-term and short-term laser heating of the examined tissue. Characterized in that: The examined pathological tissue is placed in the head of the NMR tomograph. A 3D image of the speed of spin-lattice R1 relaxation and spin-spin R2 relaxation in the examined tissue area is produced. Then, the biological material prepared in this manner is heated by means of one of the methods known in hyperthermia. Simultaneously, the heated biological tissue undergoes computer NMR tomography, while measurements are carried out of the relative changes in the spin-lattice relaxation process defined by ΔR1/R1ex, where R1 = T1-1, where T1 is the spin-lattice relaxation time, and R1ex = R1 value extrapolated at the commencement of the tissue heating process. Then, measurements are carried out of the relative speed variations of the spin-spin relaxation process defined by ΔR2/R2ex, where R2 = T2-1, where T2 is the spin-spin relaxation time, and R2ex = R2 value, extrapolated at the commencement of the tissue heating process. Then, the values of speed relaxation variations articulated in this manner are used to obtain a NMR tomographic image, where degree of blackening is proportionate to the degree of protein denaturation in the biological sample subject to hyperthermia.