Automatic system for the synthesis and chemical modification of oligomers having a predetermined sequence using soluble supporting polymers

摘要

The synthesis of oligomers in a predetermined sequence, such as peptides, oligosaccharides, oligonucleotides, etc., is very important in the sectors using those products for diagnostic and therapeutic use. The problem of their synthesis has now been solved through the use of insoluble supporting polymers which aid intermediate purification and automation of the corresponding synthesis process. The polymer in fact makes it possible to convert the intermediate purification processes into simple filtrations and washes. These systems, even though they can be automated, are of limited application for large scale production on account of the heterogeneity of the process and the requirement for large quantities of reagents. This system makes it possible to use soluble polymers which instead permit operation in a homogeneous phase. In this way a lesser quantity of reagents is required for an equal yield and increasing the scale of synthesis is not impeded by the phase heterogeneity of the process, which makes the yield from synthesis unforeseeable and renders it difficult to handle large quantities of support. The use of this system is dedicated to the large scale synthesis of natural and chemically modified oligomers, having a predetermined sequence which may or may not be stably bound to the supporting polymer, carried out using automated processes.