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METHOD AND DEVICE FOR SEPARATING AND DETECTING COMPONENTS OF A SUBSTANCE MIXTURE BY TEMPERATURE GRADIENT GEL ELECTROPHORESIS
METHOD AND DEVICE FOR SEPARATING AND DETECTING COMPONENTS OF A SUBSTANCE MIXTURE BY TEMPERATURE GRADIENT GEL ELECTROPHORESIS
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机译:用温度梯度凝胶电泳分离和检测物质混合物中成分的方法和装置
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摘要
In a process for separating and detecting constituents of a mixture of substances by temperature gradient gel electrophoresis (TGGE), a spatial temperature gradient is constructed from spatially separate temperature levels, or a temporal temperature gradient is constructed, or a temperature gradient is constructed from the spatial and temporal temperature gradients. The temperature levels used to construct the spatial temperature gradients are set by controllable heating or cooling devices. For the construction of the temporal temperature gradients, the temperature level at any point in the separating section in the separating medium can be varied with time as desired by means of controllable heating or cooling devices. A device for implementing the process with controllable heating or cooling devices for constructing temperature gradients, a hollow body arranged between the temperature levels which contains the separating medium used, and a thermostatted jacket which surrounds the hollow, are also described.
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