Enantioselective hydrolysis of ketoprofen esters by (beauveria bassiana) and enzymes derived therefrom

摘要

A process for producing substantially pure R-ketoprofen by the enantioselective hydrolysis of racemic ketoprofen choline ester is disclosed. The process utilizes either intact Beauveria bassiana hyphae or an R-specific ester hydrolase isolated therefrom. The ester hydrolase has an approximate molecular weight of 17,800 daltons and an N-terminal sequence of Ala-Pro-Asp-W-Ile-Ile-Gln-Gly-Leu-Ser-Arg-Ala-X-Asp-Gly-Gln-Asp.