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METHODS FOR PRODUCING SOLUBLE, BIOLOGICALLY-ACTIVE DISULFIDE BOND-CONTAINING EUKARYOTIC PROTEINS IN BACTERIAL CELLS
METHODS FOR PRODUCING SOLUBLE, BIOLOGICALLY-ACTIVE DISULFIDE BOND-CONTAINING EUKARYOTIC PROTEINS IN BACTERIAL CELLS
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机译:细菌细胞中可溶的,生物活性的,含二硫键的真核生物蛋白的制备方法
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摘要
Disclosed are methods of producing eukaryotic disulfide bond-containing polypeptides in bacterial hosts, and compositions resulting therefrom. Co-expression of a eukaryotic foldase and a disulfide bond-containing polypeptide in a bacterial host cell is demostrated. In particular embodiments, the methods have been used to produce mammalian pancreatic trypsin inhibitor and tissue plasminogen activator (tPA) in soluble, biologically-active forms, which are isolatable from the bacterial periplasm. Also disclosed are expression systems, recombinant vectors, and transformed host cells.
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