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METHOD AND APPARATUS FOR STUDYING THE REACTION PATTERN OF CELLS/CELL AGGREGATES DURING PERFUSION BY A TEST MEDIUM
METHOD AND APPARATUS FOR STUDYING THE REACTION PATTERN OF CELLS/CELL AGGREGATES DURING PERFUSION BY A TEST MEDIUM
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机译:用试验介质研究细胞/细胞凝集物灌注过程中反应模式的方法和装置
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摘要
the invention relates to a method for study by light microscopy, the cell reaction model of clusters of cells, e.g. cancer cells, with a high resolution in time, at the time of the infusion by means of at least one test medium in the liquid state, and the environment u consisting of, for example, cytotoxic drugs.according to the invention, the cells are introduced and clusters of cells in a small space (17) disposed in the chamber for infusion, in which they were perfused with a medium control, motility, and the reactions of cells being studied and recorded.then, the cell clusters of cells were perfused with the test medium, which results in an exchange of control medium with a time resolution in the model of motility and responses of cells and clusters of cells in the test medium as well s was investigated and recorded.it is thus possible to obtain important information on the cell shape, cell size, cell motility, cell death, cell fluorescence, etc. the invention also relates to a device useful for carrying out the process.the apparatus comprises a space (3) serving as a reservoir for the perfusion, as well as a small space (17) for the cells and clusters of cells through which the infusion is carried out.further, the apparatus comprises a microscope through which we can study the model of motility and responses of cells and cell aggregates during infusion and suction means to leave a space (17) and in another. to participate in the same space (17).
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