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METHOD FOR THE STORAGE OF ENTOMOPATHOGENIC NEMATODES.

机译:存储致病性线虫的方法。

摘要

Long-term storage of infective third stage juveniles of nematodes belonging to the genera (Steinernema) or (Heterorhabditis) is effected by mixing together an aqueous cream of the nematodes and particles of a highly water-absorptive material. The water present in the mixture is such that the mixture, after equilibrating, has a water activity in the range of from 0.80 to 0.995 (preferably 0.95 to 0.99). The preferred absorbent material is anhydrous polyacrylamide gel in the form of particles having an average mass of 0.01 gm. For optimal survival of the nematodes, the equilibrated mixture should be stored at a temperature in the range of from 1 C to 30 C, in an atmosphere which maintains the water activity of the mixture in the range of from 0.80 to 0.995.
机译:通过将线虫的水性乳膏和高吸水材料的颗粒混合在一起,可以长期保存属于线虫(Steinernema)或(线虫性线虫)的线虫感染性第三阶段幼虫。混合物中存在的水应使混合物平衡后的水活度为0.80至0.995(最好为0.95至0.99)。优选的吸收剂材料是平均质量为0.01gm的颗粒形式的无水聚丙烯酰胺凝胶。为了使线虫最佳存活,平衡后的混合物应在1 C至30 C的温度范围内的气氛中保存,该气氛应使混合物的水活度保持在0.80至0.995的范围内。

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