OLIGONUCLEOTIDES DERIVED FROM SOD FAMILY, AMPLICATION AND DETECTION METHOD OF SOD GENE

摘要

The use of the superoxide dismutase (SOD; EC 1.15.1.1) gene system as a target for the detection and differentiation of pathogenic and non-pathogenic organisms including bacteria, fungi and protozoans is described. More particularly, there are described oligonucleotides derived from the SOD gene family which oligonucleotides are capable of hybridyzing to a single-stranded nucleic acid sequence (target sequence) which is obtainable by amplifying and denaturing a part of a gene coding for a superoxide dismutase (SOD) using a polymerase chain reaction (PCR) and a pair of primers, the first primer containing the sequence 5'-AGC TTC ACC ACA GCA AGC ACC A-3' (Seq. Id. No. 1; Z205) as priming sequence and the second primer containing the sequence 5'-TCG G(T)CC GAG TTC ACG ACG(A,T) TTC CA-3' (Seq. Id. No. 2; Z212) as priming sequence. These oligonucleotides may be used in the form of primers and probes to amplify and detect target sequences derived from SOD genes, especially, from SOD genes of pathogenic and non-pathogenic organisms including bacteria (e.g. mycobacteria), fungi and protozoans.