The present invention relates to a nucleic acid encoding a glycosyltransferase, a protein encoded thereby, and a method for synthesizing oligosaccharides using the glycosyltransferase of the present invention.;In one embodiment, the glycosyltransferase of Nyseria gonorrhoea contains 5 open reading frames for 5 different glycosyltransferases. The functionally active glycosyltransferase of the present invention can be obtained by adding Gal [beta] i → 4 to GlcNAc or Glc; Adding GalNAc or GlcNAc? 1? 3 to Gal; And catalytic action such as adding? 1? 4 to Gal.;The glycosyltransferase of the present invention is preferably a Galβ1 → 4GlcNAc β1 → 3Gal β1 → 4Glc (complementary to lacto-N-neotetraose) oligosaccharides, GalNac β1 → 3Gal β1 → 4GlcNAc β1 → 3Gal β1 → 4Glc β1 → 4 Auxiliary Ganglioside) and Gal? 1? 4Gal? 1? 4Glc? 1? 4Hep-R (complementary to saccharide of glycoglycolipid) oligosaccharides.
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