METHOD 'SCREEN-ALAT-TEST' OF ALANINE AMINOTRANFERASE ACTIVITY ASSAY IN BLOOD SERUM (PLASMA)

摘要

FIELD: medicine, biochemistry. SUBSTANCE: biological fluid sample (blood plasma or serum) is taken at equal amount with substrate-buffer system that involves DL-alpha-alanine at concentration 0.33 mM, 2-oxoglutarate at concentration 3.3 mM and buffer solution, pH = 7.4. Mixture is incubated at 37 C for 15 min, not less. Then 1 mM solution of 2,4-dinitrophenylhydrazine is added to a mixture, kept at the room temperature for 20 min, not above. Then 0.4 N alkali solution is added to a mixture and kept at the room temperature for 5 min, not less. Enzyme activity is measured by colorimetry method. EFFECT: high effectiveness of enzyme activity assay.