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Dark field stimulated fluorescence microscope - has given surface of sample stimulated by zonal light beam with detection of resulting fluorescence via optical objective system
Dark field stimulated fluorescence microscope - has given surface of sample stimulated by zonal light beam with detection of resulting fluorescence via optical objective system
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机译:暗场激发荧光显微镜-给定了区域光束激发的样品表面,并通过光学物镜系统检测了产生的荧光
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摘要
The microscope is used for examination of a fluorescent material, stimulated by an illumination system providing a zonal light beam directed onto a required surface of the sample (1), with collectionof the stimulated fluorescence via an optical objective system. The stimulation light is supplied to the sample outside the optical objective system and lies below the fluorescence saturation intensity, the optical objective system having at least one filter (31,32) for blocking the stimulation light wavelength in front of the fluorescence detector (40).
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