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Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays
Use of DNA-dependent RNA polymerase transcripts as reporter molecules for signal amplification in nucleic acid hybridization assays
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机译:使用DNA依赖性RNA聚合酶转录本作为报告分子在核酸杂交测定中进行信号放大
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摘要
A polydeoxynucleotide construct is disclosed for use, in conjunction with a DNA-dependent RNA polymerase, as a signal amplifier in nucleic acid hybridization assays. The construct contains a recognition sequence for a target oligonucleotide, a promoter sequence for a DNA- dependent RNA polymerase, and a polymerase template. A method of use for this construct in hybridization assays is also disclosed. The method involves formation of a hybridization complex comprising the construct and the target sequence; addition of a polymerase which is specific for the promoter in the construct; and quantification of the resulting RNA transcripts.
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