DNA sequence which binds transcriptional regulatory proteins activated in response to various cytokines and uses thereof

摘要

An oligonucleotide comprising the DNA sequence TTTCCNGGAAA (SEQ ID NO:1) in single stranded or double stranded form, or a derivative thereof, binds to transcriptional regulatory proteins induced by cytokines, growth factors or hormones. Such oligonucleotides are useful in detecting, identifying or purifying transcriptional regulatory proteins. Recombinant DNA constructs comprising the oligonucleotide sequence operably linked to a promoter and a reporter DNA sequence and cells transfected therewith are also disclosed. Also disclosed are DNA- binding proteins other than p 91 which are inducible in a cell by a cytokine, growth factor or hormone, and which, when tyrosine phosphorylated, bind to the above oligonucleotide DNA sequence.