Antibodies that bind to the CD4 molecule conformation induced by the binding of human immunodeficiency virus has changed

摘要

(57) the surface of the [Abstract] CD4 ⁺ cells, binds to CD4 altered form of conformation induced upon contact its envelope protein (eg, gp120) of human immunodeficiency virus or cells thereof (HIV) I disclose an antibody that does not substantially bind to CD4 pristine on the surface of a cell prior to contact with the envelope protein Suruga, or HIV. Preferred antibodies of the present invention is a 3-51 and 3-47 monoclonal Fab fragments. Pharmaceutical compositions using the antibody of the present invention, as well as I disclose diagnosis and methods of treatment and screening. Identification of agents that induce or prevent detection of CD4 altered form of conformation on the cell surface, the formation of CD4 altered form of this conformation upon gp120 binding, and antibodies of the invention, CD4 ⁺ is useful for the prevention of infection by HIV in a cell. The surface of CD4 ⁺ cells, molecules expressing one epitope also, in the invention, at least it is expressed CD4 on the altered form of conformation induced upon contact with the envelope proteins or HIV in the cell are included. Can be induced in the patient an antibody response using such molecules, to prevent infection by HIV in a cell in a patient and to generate antibodies of the present invention.