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APPARATUS FOR ASSAY OF LIQUID SAMPLE, APPARATUS FOR DNA AMPLIFICATION ASSAY AND METHOD THEREFORE

机译:液体样品的测定装置,DNA扩增测定的装置及其方法

摘要

PROBLEM TO BE SOLVED: To provide the subject apparatus of a small volume having no head space and free from the occurrence of vaporizing and condensing of a reagent, etc., storing a liquid sample in the apparatus by suctioning it into a capillary section between a sample well and an optical window member and tightly closing it with a closing body. ;SOLUTION: Into a capillary section 54 between the upper surface of the bottom wall of a sample well 14 and an optical window member 20, a liquid sample 60 is introduced through a gap 58 with a pipette 66, etc., to be suctioned and the sample is tightly closed by covering with a closing body 18. A reagent in a spot 56, i.e., a dried reagent, which is to be used for homogeneous amplification and fluorescence polarization assay of nucleic acid and adhered on the central position of the upper surface of the bottom wall of the sample well 14, is hydrated to start the objective amplification and assay reactions.;COPYRIGHT: (C)1998,JPO
机译:解决的问题:提供一种小体积,没有顶部空间并且没有发生试剂汽化和凝结等现象的主题设备,通过将液体样品抽吸到设备之间的毛细管部分中,将液体样品存储在设备中。样品孔和一个光学窗口部件,然后用关闭体将其紧密关闭。 ;解决方案:将液体样品60通过带有吸液管66等的间隙58引入样品池14底壁上表面和光学窗部件20之间的毛细管部分54中,以进行抽吸和样品通过用封闭体18覆盖而紧密封闭。斑点56中的试剂,即干燥的试剂,将用于核酸的均相扩增和荧光偏振测定,并粘附在上部样品的中心位置将样品孔14底壁的表面水合以开始客观扩增和测定反应。;版权所有:(C)1998,日本特许厅

著录项

  • 公开/公告号JPH10127268A

    专利类型

  • 公开/公告日1998-05-19

    原文格式PDF

  • 申请/专利权人 BECTON DICKINSON & CO;

    申请/专利号JP19970279939

  • 发明设计人 COTTINGHAM HUGH V;

    申请日1997-09-26

  • 分类号C12M1/00;C12M1/22;C12M1/26;G01N21/75;G01N21/78;

  • 国家 JP

  • 入库时间 2022-08-22 03:08:21

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