REMOVAL OF ANTIGENIC INGREDIENT, NONANTIGENIC PEPTIDE COMPOSITION, NONANTIGENIC STABILIZER AND PHYSIOLOGICALLY ACTIVE SUBSTANCE

摘要

PROBLEM TO BE SOLVED: To obtain a physiologically active substance useful for a nonantigenic stabilizer and a medicine, etc., by treating peptide composition derived from collagen or gelatin according to a reversed phase chromatography, a hydrophobic chromatography, etc., and removing an antigenic ingredient. SOLUTION: A peptide composition obtained by digesting collagen or gelatin with a protease enzyme such as a collagenase, a pepsin, a trypsin or a papain is passed through a column for a high-performance liquid chromatography filled with a support for a reversed phase chromatography in which a substrate is a silica gel to adsorb a peptide thereon to elute a peptide fraction without containing an antigenic or an allergenic ingredient by a linear gradient with a 0-30% methanol or fractionated with a high-performance liquid chromatographic system using a column filled with a support for a hydrophobic chromatography in which the substrate is agarose beads or the peptide composition is treated by both thereof to remove an antigenic ingredient. Thereby, the objective antigenic stabilizer and physiologically active substance are obtained.