TYROSINASE GENE OF SHIITAKE (LENTINUS EDODES)

摘要

PROBLEM TO BE SOLVED: To obtain the subject new gene consisting of a DNA having a specific amino acid sequence and encoding a protein bringing a tyrosinase activity, and used for the production of an enzyme useful for an enzymatic browning of cacao and coffee beans, a production of melanin, etc. ;SOLUTION: This tyrosinase gene of Shiitake (Lentinus edodes) is a new one encoding a protein having an amino acid sequence expressed by the formula including sequences in which one or plural amino acids are deficient, substituted or added to the above sequence, bringing a tyrosinase activity, and useful in the production of an enzyme capable of oxidizing a monophenolic and a polyphenolic compound related to a melamine synthesis in a body, and used in an enzymatic browning of cacao and coffee beans in a food industry and a production of melanin in a medicinal industry. The gene is obtained by extracting a mRNA from Shiitake belonging to the genus Lentinus, synthesizing cDNA by using the mRNA, and performing a PCR by using a primer consisting of a partial amino acid sequence of the tyrosinase.;COPYRIGHT: (C)1998,JPO