Cryopreservation of cultured epithelial cell sheet

摘要

The viability of cohesive sheets of cultured epithelial cells such as keratinocytes used as wound dressings is maintained or improved by storage at a temperature within the range of 8°-25° C., preferably 13°-23° C., for periods greater than 8 hours, preferably up to 26 hours. This method and the resulting product permits a significant increase in the shipping radius of cultured dressings manufactured in a central facility, and increases flexibility in scheduling of shipments and surgical procedures.