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A method for the selective degradation of milk protein, in particular for the selective hydrolysis of casein / caseinate in the presence of other milk proteins, in particular whey proteins.
A method for the selective degradation of milk protein, in particular for the selective hydrolysis of casein / caseinate in the presence of other milk proteins, in particular whey proteins.
A method is presented for producing a casein/caseinate hydrolysate stripped of immunogenic proteins. The method comprises treating an aqueous solution, such as milk, comprising casein/caseinate and immunogenic protein other than casein with a protease that selectively hydrolyzes casein. After the casein/caseinate has been hydrolyzed, the casein/caseinate hydrolyzate is separated by membrane ultrafiltration from unhydrolyzed immunogenic protein and recovered. The resulting hydrolyzate is essentially free of antigenic components of the ABBOS peptide and bovine serum albumin. The casein selective protease is selected from cell wall proteinase of Lactococcus lactis subsp. Cremoris Wg2, a Clostridium histolyticum collagenase and a Bacillus polymyxa dispase.
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