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EXPRESSION OF HAEMOPHILUS INFLUENZAE TYPE B OUTER MEMBRANE PROTEIN P2 IN G.COLI
EXPRESSION OF HAEMOPHILUS INFLUENZAE TYPE B OUTER MEMBRANE PROTEIN P2 IN G.COLI
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机译:流感嗜血杆菌B型外膜蛋白P2在大肠杆菌中的表达
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摘要
The present invention relates, in general, to a method of expressing the outer membrane protein P2 from Haemophilus influenzae type b (Hib-P2) and fusion proteins thereof. In particular, the present invention relates to a method of expressing the outer membrane protein P2 from Haemophilus influenzae type b or fusion protein thereof in E. coli wherein the Hib-P2 protein or fusion protein comprises more than 2% of the total protein expressed in E. coli. The invention also relates to a method of purification and refolding of Hib-P2 protein and fusion protein thereof.
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