Method for increasing the production of penicillin G (benzylpenicillin) in Penicillium chrysogenum by expression of the pcl gene.

摘要

PROCEDURE FOR INCREASING PRODUCTION OF PENICILLIN G (BENZYLPENICILLIN) IN Penicillium chrysogenum BY GENE EXPRESSION PCL. GENE PLC coding for the enzyme phenylacetyl-CoA ligase. Using techniques of DNA recombinant INTRODUCE GENE PCL OBTAINED Pseudomonas putida UY characterized by the sequence SEQ ID NO: 1, yielding MUTANT TRANSFORMED CECT 20192, exhibiting increased production of a minimum of 10% Penicillin G, WITH RESPECT TO THE STRAINS untransformed control.