NON RADIOACTIVE RECEPTOR ASSAY SUITABLE FOR QUANTITATIVE AND QUALITATIVE ANALYSIS OF TRACE AMOUNTS OF RECEPTOR BINDING ANALYTE

摘要

A method for quantitatively and/or qualitatively assaying an analyte in asample, said analyte being a receptor binding compound, said method having lowdetection limits equivalent to those of radioreceptor assays, said methodcomprising the steps of a) contacting the sample with material comprising areceptor for said analyte in order for receptor-analyte binding to occur andb) further contacting the sample with a detectable ligand for the receptor inorder for receptor-ligand binding to occur, followed by c) separating theresulting receptor bound and free fractions, d) subjecting the receptor boundfraction to dissociating conditions releasing the ligand from the receptor ande) assaying for the dissociated ligand in a manner known per se for thedetection of the detectable ligand.