METHODS FOR SCREENING OF TEST COMPOUNDS FOR INHIBITING BINDING OF A CD4-HIV-1 COMPLEX TO HIV-1 SECOND RECEPTORS

摘要

A method for the screening of a test compound for inhibiting the binding of a CD4-HIV-1 complex to HIV-1 second receptors, comprising: (a) preparing a magnetic ligand by mixing a magnetic, CD4-containing substrate with HIV-1, (b) mixing the magnetic ligand from step (a) with a test compound, (c) adding cells that express the HIV-1 second receptors to the mixture from step (b), (d) separating cells with bound magnetic ligands from cells without bound magnetic ligands by contact with a magnetic separator, and (e) quantifying the cells with bound magnetic ligands and quantifying the cells without bound magnetic ligands.