The invention relates to methods of detecting the: genus Listeria; species Listeria monocytogenes; and species Rhodococcus coprophilus. All primers that react with the named species, but do not cross react with related or unrelated species of bacteria are claimed. The preferred primers for the: genus species Listeria monocytogenes; Listeria; and species Rhodococcus coprophilus; come from the: Listeriolysin O (HylA); 23s rRNA subunit; and 16s rRNA, genes respectively.
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