HYDROPHILIC POLY-(N-SUBSTITUTED)ACRYLAMIDE GELS AND THEIR USE IN ELECTROKINETIC AND CHROMATOGRAPHIC METHODOLOGIES

摘要

the electrokinetic separation and chromatographic matrices based on a unique class of mono - and di substituted acrylamides (on the amido nitrogen (e.g. hu00e9thanol acrylamido - n '- ethoxy) ethanol acrylamido - n, n - (ethoxy)have the following advantages: a) strong resistance to alkaline hydrolysis, separation of most of the areas in the basic ph values); (b) high hydrophilicity and (c) the higher porosity, the higher value of mr. monomers.such matrices can be prepared as a "chemically cross-linked gels" or "physical gels, which is composed of" son "of monofunctional monomer in the absence of cross-linking at concentrations above the level of the mixture.in addition, it is possible to increase the porosity of the gel in the presence of agglomerating agents polymerising side (for example, polyethylene glycol - 6 - 20000 da), thus obtaining matrices that allow better resolution of proteins and fragments of mr is high nucleic acid; and polymerising the matrix in the presence of a gradient of the sintering agent sideit is possible to obtain a gradient of porosity in a constant proportion of monomers in the gel.finally, the photopolymu00e9risation (e.g., methylene blue, and a couple of redox and diphenyliodonium chloride, sodium toluu00e8nesulfinate and light) in place of the standard red / ox catalysts, prevents the formation of oxides is present in the solution of amines the polymerization.