Deacytelase genes for the production of phosphinotricin or phosphinotricin-alanyl-alanile, process for their isolation, and uses thereof

摘要

Claimed is an isolation method for deacetylase genes (DEAs) from microorganisms by producing a gene bank of a mutant carrying the phosphinothricin-N-acetyltransferase gene (pat) and isolating DEA-carrying clones by phosphinothricin (PTC) and phosphinothricyl-alanyl-alanine (PTT) sensitivity. Also claimed are (a) the identification of tissue-specific promoters by putting a DEA gene under the control of the promoter to be tested and observing specific cell death, and (b) a positive selection system for the determn. of cloning or transposition by insertion-inactivation by selecting in combination with either the pat-gene with PTT or PTC and the DEA, or DEA with N-acetyl PTT and -PTC those cells in which the DEA gene was inactivated.