Method for Purifying Active Site Collagenase-3 Expressed in E. Coli

摘要

The present invention relates to a method for purifying the active site collagenase-3 expressed in Escherichia coli, crushing the prepared E. coli cells in which the active site collagenase-3 is expressed in an inclusion body form, recovering the inclusion body, and then washing the urea. According to the purification method of the present invention comprising dissolving in solution, dialysis by cation exchange chromatography, gel filtration chromatography and restitution, active site collagenase-3 protein can be obtained in high yield.