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Ethylenediamine-N, N'-disuccinic acid: a protein having ethylenediamine lyase activity and a gene encoding the same
Ethylenediamine-N, N'-disuccinic acid: a protein having ethylenediamine lyase activity and a gene encoding the same
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机译:乙二胺-N,N'-二琥珀酸:具有乙二胺裂解酶活性的蛋白质和编码该蛋白质的基因
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摘要
The present invention relates to an ethylenediamine-N, N'-disuccinic acid: DNA comprising a nucleotide sequence encoding a polypeptide having ethylenediamine lyase activity, wherein the polypeptide is at least an amino acid sequence represented by SEQ ID NO: 1 or in this amino acid sequence. DNA having an amino acid sequence in which one amino acid has been deleted, substituted, or added, a recombinant plasmid containing the DNA, a host transformed with the recombinant plasmid, or a diaminoalkylene-N, N 'using the transforming host It relates to a process for preparing disuccinic acid. In addition, the present invention has an amino acid sequence represented by SEQ ID NO: 1 or an amino acid sequence in which at least one amino acid is deleted, substituted or added in this amino acid sequence, and has an ethylenediamine-N, N'-disuccinic acid: ethylenediamine lyase activity. It relates to a polypeptide having.
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机译:本发明涉及一种乙二胺-N,N′-二琥珀酸:包含编码具有乙二胺裂解酶活性的多肽的核苷酸序列的DNA,其中该多肽至少是由SEQ ID NO:1表示的氨基酸序列或在该氨基酸中酸顺序。本发明涉及一种具有氨基酸序列的DNA,该氨基酸序列中缺失或取代了1个氨基酸的重组质粒,含有该DNA的重组质粒,用该重组质粒转化的宿主,或使用该转化宿主的二氨基亚烷基-N,N'。一种制备二琥珀酸的方法。另外,本发明具有由SEQ ID NO:1表示的氨基酸序列或在该氨基酸序列中至少一个氨基酸被缺失,取代或添加的氨基酸序列,并且具有乙二胺-N,N '-二琥珀酸:乙二胺裂解酶活性。它涉及具有的多肽。
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