Method for preparing lyophilized cells with excellent shelf life and freeze-dried protective composition for same

摘要

In the method for producing lyophilized cells for long-term preservation of microorganisms, 20 to 70 parts by weight of skim milk, 50 to 100 parts by weight of lactose, 30 to 100 parts by weight of galactose, trehalose based on 100 parts by weight of dry cells By adding a composition consisting of 20 to 100 parts by weight of the freeze-dried protective agent to the concentrated culture solution to improve the number of viable cells in freeze-dried cells and the stability of the freeze-dried cells can be increased.