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USE OF BETA-SHEET FORMING AMINO ACID LEADER SEQUENCE FOR THE PRODUCTION OF PROTEINS AND PEPTIDES
USE OF BETA-SHEET FORMING AMINO ACID LEADER SEQUENCE FOR THE PRODUCTION OF PROTEINS AND PEPTIDES
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机译:使用形成贝他表的氨基酸铅序列生产蛋白质和肽
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摘要
The present invention relates to an effective method for preparing a polypeptide or a precursor thereof using the following general formula.;A-L-B A: Fusion partner (amino acid sequence of all or part of protein forming beta-sheet structure);L: Linker (arranged to fit codons);B: polypeptide or polypeptide precursor;More specifically, the present invention specifically relates to genes encoding fusion proteins consisting of all or a portion of tumor necrosis factor and the polypeptide to be expressed, vectors comprising the genetic factors, microorganisms transformed with the vectors, and fusion proteins. It relates to the preparation and purification method of.
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