Method for producing hepatitis B virus X protein in Escherichia coli and monoclonal antibody against X protein, hybridoma cell line producing same and method for producing same

摘要

The present invention is a regulatory protein among four proteins constituting Hepatitis B Virus (hereinafter abbreviated as “HBV”), and is an X protein which is estimated to be directly involved in the mechanism of HBV-induced hepatitis transition to liver cancer. Expression plasmid that can be mass-produced in Escherichia coli and method of manufacturing X protein using the same, and X protein which can be useful for diagnosing hepatitis virus liver disease, hepatitis and liver cancer by measuring the level of X protein in blood It relates to a mouse monoclonal antibody, a hybridoma cell line producing the same and a method for producing the same.