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A maltogenic amylase gene, a recombinant molecule containing it, a transformant having the same, and an enzyme produced therefrom

机译:麦芽糖淀粉酶基因,包含它的重组分子,具有该麦芽糖淀粉酶的转化体以及由此产生的酶

摘要

The present invention relates to a maltose-producing amylase gene isolated from Bacillus stearothermophilus KCTC1044BP, which has a genetic map as shown in FIG. 1 and has a molecular weight of about 62000 Dalton as measured by 10% SDS-PAGE and isoelectric focusing (IEF ), The maltose-producing amylase produced by this gene acts on fluoran to produce mainly pannocose, decomposes starch to mainly produce maltose, and decomposes? -Cyclodextrin , And a maltogenic amylase gene. The present invention also relates to a recombinant molecule comprising said gene, a transformant having said recombinant molecule, and an enzyme produced therefrom.
机译:本发明涉及从嗜热脂肪芽孢杆菌KCTC1044BP分离的产生麦芽糖的淀粉酶基因,其基因图如图1所示。如图1所示,通过10%SDS-PAGE和等电聚焦(IEF)测得的分子量约为62000道尔顿。由该基因产生的产生麦芽糖的淀粉酶作用于荧烷上以主要产生潘诺糖,分解淀粉以主要产生麦芽糖,以及分解? -环糊精,和一种产麦芽糖淀粉酶基因。本发明还涉及包含所述基因的重组分子,具有所述重组分子的转化体以及由此产生的酶。

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