VECTOR PMT440 AT POSITIVE SELECTION FOR FOREIGN DNA FRAGMENTS CLONING

摘要

FIELD: molecular biology, genetic engineering. SUBSTANCE: vector has barnase structural gene subcloned from Bacillus amyloliquefaciens under control of synthetic tac-promoter; universal polylinker of plasmid pHC19 consisting of 45 b. p. inserted in barnase gene instead of valine-36; phoA-signal sequence from Escherichia coli; gene of specific barnase inhibitor - barstar under its own promoter; plasmid pHC19 fragment including replication start site ori, gene A_mp r.. Restriction sites are located at the following positions from replication start point of plasmid DNA, b. p.: Eco RI 300; SacI 306; KpuI 312; SmaI 316; Bam HI 321; SalI 333; PstI 339; SphI 345, and HindIII 351. Vector size is 2936 b. p., its capacity is 6 t.b.p. Vector provides positive selection of cloning fragments of foreign DNA in different strains of E. coli. EFFECT: improved method of selection. 1 dwg