STRAIN OF BACTERIUM PHOTOBACTERIUM PHOSPHOREUM - A PRODUCER OF RESTRICTION ENDONUCLEASE RECOGNIZING AND SPLITTING NUCLEOTIDE SEQUENCE 5'-GG(T/C)(AG)CC-3'

摘要

FIELD: biochemistry, genetic engineering, biotechnology. SUBSTANCE: invention proposes new strain of Photobacterium phosphoreum VKPM B-6687 as a producer of restrictase Pph 14 1 isolated from sea water as a result of restrictase producers screening. Enzyme yield is 10000 U/g wet biomass, specific activity of the end enzyme is 20000 U/mg. Restrictase Pph 14 1 is an isoschizomer of restrictase HgiH 1 and can replace the latter in all genetic engineering works. EFFECT: new strain indicated above, increased yield and specific activity. 1 dwg