DNA FRAGMENTS ENCODING HUMAN ERYTHROPOIETIN, RECOMBINANT DNAS, CELL LINE, RECOMBINANT O-GLYCOSYLATED HUMAN ERYTHROPOIETIN, PHARMACEUTICAL COMPOSITION

摘要

FIELD: biotechnology, medicine. SUBSTANCE: genome DNA fragments from human fetal liver and the corresponding fragments to DNA providing high expression levels of erythropoietin are cloned in mammalian cells. Cell line CHO - a producer of recombinant erythropoietin (CHO EPO Cla 44.02-7) is obtained. As a result of expression of the cloned DNA fragments in mammalian cells O-glycosylated human erythropoietin is obtained at specific activity 200 000 U/mg, not less, and pharmaceutical composition using the indicated recombinant erythropoietin as an active component. EFFECT: improved method of preparing, increased specific activity of erythropoietin. 12 cl, 11 tbl, 12 dwg