A method for producing an endoinulinase producing microorganism, a method for the production of endoinulinase, as well as a method for the production of oligofructosiden from insulin and method for the detection of endoinulinase - activity

摘要

The invention relates to a method for obtaining an endoinulinase-producing microorganism, comprising the following steps: collecting earth and/or tissue samples in the root area of inuline-storing plants; selecting microorganisms using inuline as the only carbon source, by adding an inulin-containing agent during a determined period; separating and culturing said selected microorganisms. The invention also relates to a microorganism having strictly an endoinulinase-activity, as well as a method for detecting such an activity, comprising the following steps: marking said inulin with a marker substance; incubating the marked inulin with inulase during a determined period; separating the non-converted marked inulin; determining the charge extinction at 592 nm.