Genes for butyrobetaine/crotonobetaine-l-carnitine metabolism and their use for the microbiological production of l-carnitine

摘要

An isolated DNA fragment including the genes bcoC, bcoA/B, bcoD coding for the enzymes for L-carnitine biosynthesis in - butyrobetaine/crotonobetaine metabolism and, additionally, including the gene bcoT coding for a potential transport protein in - butyrobetaine/crotonobetaine metabolism. Also, any vector which includes an isolated DNA fragment including the genes bcoC, bcoA/B, bcoD coding for the enzymes for L-carnitine biosynthesis in - butyrobetaine/crotonobetaine metabolism and, additionally, including the gene bcoT coding for a potential transport protein in - butyrobetaine/crotonobetaine metabolism. Also, biotechnological process for the production of L-carnitine characterized in that crotonobetaine and/or -butyrobetaine is fermented in the presence of a suitable carbon and nitrogen source using a microorgansim and L-carnitine is isolated.