ANALYSIS METHOD FOR EXCITATORY AMINO ACID EMITTED FROM BRAIN SLICE

摘要

PROBLEM TO BE SOLVED: To analyze the emission of excitatory amino acid of a brain nervous system in real time in two dimensions by bringing a glutamic acid receptor channel manifestation cell into touch with a brain slice and detecting a channel function of a glutamic acid receptor of the cell. ;SOLUTION: For instance, a cDNA fragment coding an NMDA glutamic acid receptor sub unit is embedded in an animal cell manifestation vector holding an inductive promotor, introduced in an animal cell together with a vector holding a chemical solution-resistant gene and incubated, whereby a chemical solution-resistant cell strain is obtained. A glutamic acid receptor channel manifestation cell that can form an ion channel is thus obtained. A brain slice is brought in touch with the cell. A concentration rise of ions in the cell resulting from the activation of a receptor by an excitatory amino acid emitted from the brain slice is detected by a fluorescent microscopic image-processing apparatus. Accordingly, the emission of the excitatory amino acid from the brain slice can be analyzed in real time in two dimensions.;COPYRIGHT: (C)1999,JPO