PROBLEM TO BE SOLVED: To surely discriminate a dry leaf of excellent quality, a rapidly dried leaf of low quality and a steamed leaf while reflecting the difference in content components relating to a flavor and a taste. ;SOLUTION: A brown pigment in a dry leaf of a leaf tobacco is extracted using deionized water, and the resulting extract is injected into a liquid chromatograph equipment in which a reversed phase column is used for a separation column and in which a deionized water/acetonitrile system solvent containing ammonium acetate is used for a separation solvent, being separated in the condition that acetonitrile concentration is increased in five steps. A normal leaf, a rapidly dried leaf and a steamed leaf are discriminated using as a index the ratio between (a first peak quantity + a second peak quantity) and (a fourth peak quantity + a fifth peak quantity) in the resulting chromatograph.;COPYRIGHT: (C)1999,JPO
展开▼