Mass production method of bacteria-eating nematode Aferenkusu-Abene

摘要

PROBLEM TO BE SOLVED: To enable the mass production of mycophagy nematode Aphelenchus abene by subjecting the solid medium consisting of plant-base industrial waste or by-products to sterilization by heating, then inoculating the medium with feed filamentous fungi and culturing the fungi. ;SOLUTION: The solid medium of pH 5.2 is obtd. by mixing, for example, 10.0g (wet weight) paper pulp and 10.0g (the same) beer-less in a glass container of 500ml volume, loading the mixture into an autoclave, sterilizing the mixture by heating for 30 minute at 120°C and then cooling the mixture. The medium is inoculated with the filamentous fungus Botrytis cinerea formed by axenic culture and the fungi are cultured for 3 days. Separately, 50 heads of these nematodes are immersed for 30 minutes in 100ppm streptomycin liquid to sterilize the body surfaces. The medium where the filamentous fungi described above are propagated for 3 days is inoculated with these nematodes and are cultured for 3 weeks, by which about 4 million heads of the nematodes per 8.4g dry weight of the medium are produced.;COPYRIGHT: (C)1998,JPO