Measurement modulo null of degree of deacetylation conversion of chitin and

摘要

PURPOSE:To easily and accurately determine the deacetylation degree of chitin and chitosan by completely decomposing chitin or chitosan with an enzyme to the constituent monosaccharides and quantitatively determining the monosaccharides. CONSTITUTION:Chitin or chitosan is decomposed into monosaccharides (Nacetyl glucosamine and glucosamine) by adding exo-type beta-D-glucosaminidase in combination with beta-N-acetylhexosaminidase (or beta-N-acetylglucosaminidase) and, as necessary, chitosanase, chitinase, etc., to a solution of chitin or chitosan. The formed monosaccharides are quantitatively determined by high performance liquid chromatography, etc., to determine the objective deacetylation degree.