Method is used to prepare the compound, 12 acetylenase of complementary DNA sequential coding Δ and its use and organism transform with acetylenase complementary DNAs of acetylene

摘要

In the present invention, describe a kind of preparation method, compound for acetylene, wherein preparation process linoleate is changed into crepenynic acid by 12 acetylenase of crepis alpina Δs according to amino acid sequence list 2, further describing is a complementary DNA sequence, crepis alpina Δ 12 acetylenase of the coding according to sequence list 2 comprising a nucleotide sequence. It is required that being also to use to be used to transformation be selected to include oil crops, the sequence of the organism of a group of the yeast and mold of oleogen. In addition, the present invention relates to the organism transforms with acetylenase complementary DNAs.