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A method for assessing the adequacy of clinical specimens for hybridization assays and kits for carrying out the assessment
A method for assessing the adequacy of clinical specimens for hybridization assays and kits for carrying out the assessment
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机译:评估用于杂交测定的临床标本是否足够的方法以及用于进行评估的试剂盒
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摘要
The adequacy of clinical specimens for nucleic acid hybridization tests is determined by a method comprising the steps of (a) taking a representative sample from the specimen; (b) applying the sample, without intermediate treatment, to a gel having dispersed therein a nucleic acid staining agent such that the staining agent binds to the nucleic acids in the sample to produce a visually detectable product; and (c) visually determining the amount of staining agent bound to the sample and comparing this to the result obtained for a known concentration of deoxyribonucleic acid and staining agent in order to determine the approximate number of cells in the sample. Reagents and apparatus for performing the method may be packaged in kit form.
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