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PROCESS FOR PREPARING ATTENUATED VARICELLA ZOSTER VIRUS (VZV) VACCINE
PROCESS FOR PREPARING ATTENUATED VARICELLA ZOSTER VIRUS (VZV) VACCINE
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机译:减毒水痘带状疱疹病毒(VZV)疫苗的制备过程
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摘要
A process for culturing cells in monolayer which comprises : a. seeding a culture vessel with a cell stock in a minimal or rich medium and allowing the cells to plant for about 24-72 hours; b. removing the medium from the culture of step a. and replacing with fresh, rich medium, supplemented with an opitimized concentration of lipids and optionally also including a serum supplement; c. growing the culture of cells in rich medium supplemented with lipid; d . optionally replacing the medium of Step c. with fresh medium containing no lipid supplement, after about 24-72 hours growth.
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