DNA ENCODING 3-PHOSPHOGLYCERATE DEHYDROGENASE, EXPRESSION VECTORS AND CELLS FOR BIOSYNTHESIS OF SERINE AND SERINE-RELATED PRODUCTS

摘要

DNA encoding 3-phosphoglycerate dehydrogenase (PGD) with reduced sensitivity to inhibition by serine in comparison to wild-type PGD. The DNA encodes PGD which has at least some level of enzymatic activity useful for biosynthesis, and which retains that activity at higher serine levels than does the (unmodified) wild-type PGD. Also disclosed are: PGD having the amino acid sequence of the above- described engineered DNA; expression vectors comprising the engineered DNA and regulatory DNA positioned and oriented for expression of the engineered DNA in a host expression system; cells comprising such expression vectors; and methods for producing serine or a serine-derived product by culturing such cells.