METHOD FOR ISOLATION OF HUMAN NECROSIS FACTOR (TNF), HUMAN TNF, A MIXTURE CONTAINING TNF, A VARIANT OF TNF, DNA CODING TNF, A CELL TRASNSFORMED WITH DNA AND PHARMACEUTICAL COMPOSITION

摘要

A method for isolating tumor necrosis factor (TNF) from a heterogeneous mixture with other proteins comprising adsorbing tumor necrosis factor (TNF) from such mixture onto at least one silicate, controlled pore glass, alkyl Sepharose, or particles of an anion exchange resin having substantially uniform particle size and thereafter eluting the tumor necrosis factor (TNF). There is further disclosed a human TNF-containing mixture, a TNF variant, TNF encoding DNA, a cell transformed by this DNA, and a pharmaceutical composition for treating tumors and containing human non-glycolysated TNF and human interferone, in particular tau-interferone.