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Zoogloeal and hyphomicrobium spp. nucleic acids
Zoogloeal and hyphomicrobium spp. nucleic acids
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机译:人畜共患菌和疏水菌属。核酸
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摘要
The invention provides two new zoogloeal strains, mz1t and mz2t. The invention provides an isolated nucleic acid consisting of the nucleic acid of SEQ ID NO:1. Examples of nucleic acids of mz1t include SEQ ID NO:2 and SEQ ID NO:3. An example of a nucleic acid of mz2t is SEQ ID NO:4. The invention also provides an isolated nucleic acid consisting of the nucleic acid of SEQ ID NO:5. A method of detecting the presence of zoogloeal clusters in a wastewater sample is provided, comprising: a) contacting RNA from a sample of the wastewater with a nucleic acid comprising the nucleic acid of SEQ ID Nos:1,2,3,4 or 5 under conditions that permit specific hybridization; and b) detecting the presence of hybridization, the presence of hybridization indicating the presence of zoogloeal clusters. A new Hyphomicrobium spp. strain, designated M3, is provided herein. The invention provides novel nucleic acids of a Hyphomicrobium sp. M3. For example SEQ ID NO:6 and SEQ ID NO:7 rDNAs of M3. SEQ ID Nos:8, 9, 10, 11, 12, 13 and 14 are presumptive Hyphomicrobium spp. sequences. SEQ ID NO:15 is a consensus sequence from 6m3 and 3m3 (Hyphomicrobium sp. m3 isolated from sludge. A method of detecting the presence of a Hyphomicrobium species in a wastewater sample as described above, using a nucleic acid comprising the nucleic acid of SEQ ID NO:17 is also provided. The nucleic acids of SEQ ID NO:6, SEQ ID NO:7, SEQ ID NO:8, SEQ ID NO:9, SEQ ID NO:10, SEQ ID NO:11, SEQ ID NO:12 SEQ ID NO:13, SEQ ID NO:14 and SEQ ID NO:15 can also be utilized in whole or as fragments in the detection method for Hyphomicrobium spp.
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机译:本发明提供了两种新的人球菌菌株,mz1t和mz2t。本发明提供了由SEQ ID NO:1的核酸组成的分离的核酸。 mz1t的核酸的实例包括SEQ ID NO:2和SEQ ID NO:3。 mz2t的核酸的实例是SEQ ID NO:4。本发明还提供了由SEQ ID NO:5的核酸组成的分离的核酸。提供了一种检测废水样品中的人兽共体簇的存在的方法,其包括:a)使废水样品中的RNA与包含SEQ ID Nos:1、2、3、4或5的核酸的核酸接触。在允许特异性杂交的条件下; b)检测杂交的存在,杂交的存在表明动物小球簇的存在。一个新的Hyphomicrobium spp。本文提供了命名为M3的菌株。本发明提供了Hyphomicrobium sp。的新核酸。 M3。例如,M3的SEQ ID NO:6和SEQ ID NO:7 rDNA。 SEQ ID Nos:8、9、10、11、12、13和14是推定的Hyphomicrobium spp。序列。 SEQ ID NO:15是从6m3和3m3(从污泥中分离的Hyphomicrobium sp.m3)的共有序列。如上所述,使用包含SEQ ID NO:1的核酸的核酸检测废水样品中Hyphomicrobium种类的存在的方法。还提供了ID NO:17,核酸为SEQ ID NO:6,SEQ ID NO:7,SEQ ID NO:8,SEQ ID NO:9,SEQ ID NO:10,SEQ ID NO:11,SEQ ID NO: NO:12 SEQ ID NO:13,SEQ ID NO:14和SEQ ID NO:15在Hyphomicrobium spp的检测方法中也可以整体或作为片段使用。
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