Quantitative enzyme immunoassay method to detect adulteration of milk from sheep milk of another species.

摘要

METHOD immunosorbent QUANTITATIVE DETECTION OF THE PRESENCE OF MILK OF DIFFERENT SPECIES IN MILK SHEEP, comprising: a) depositing a specific anti-PROTEIN DAIRY ON wells of a polystyrene plate OR OTHER MATERIAL PLASTIC capable of binding protein, B) washing away unbound antibodies to the plate; C) incubating with a solution of ovalbumin and then wash; D) applied to each well SIGN A PLATE OF MILK TO ANALYZE or master; E) washing to remove proteins which have not reacted with antibodies; F) incubating each well of the plate LACTEA anti-protein immunoglobulin, directed against the same protein as used in step "A", combined with alkaline phosphatase PEROXINAS O diluted in normal saline AT ROOM TEMPERATURE; G) washing to remove labeled antibodies NOT have reacted; H) incubating each well with a substrate of the enzyme for REVEALED, I) measuring the absorbance of each application because POCILLO.TIENE to determine the presence and quantitate MILK MILK different species and pecorino.