Use of capillary electrophoresis to determine the amount of protein component concentration and the total protein in fluids.

摘要

A method for quantifying PROTEIN IN COMPLEX SAMPLES USING ELECTROFORESIA CAPILAR can be used both to determine the concentration of a protein in a sample as TOTAL CONCENTRATION TO DETERMINE PROTEIN IN THE SAMPLE. IN GENERAL, THE METHOD FOR DETERMINING THE CONCENTRATION OF A PROTEIN MARKER includes: (1) adding a known amount of an internal standard compound to a sample containing at least one protein, the internal standard compound selected from the group comprising benzoic acid substituted with at least one halogen atom, producing a detector signal relative to its concentration and being capable of electrophoretic separation of the protein; (2) SUBMISSION OF SAMPLE AND INTERNAL STANDARD COMPOUND A ELECTROFORESIA CAPILAR FOR EACH SEPARATE PROTEIN AND INTERNAL STANDARD AND OTHER COMPOUND components in the sample; (3) DETECTOR signal measurement produced by the internal standard compound and a detector signal produced by the protein to determine a ratio of protein signal to the signal INTERNAL STANDARD; And (4) determining the concentration of protein in the sample from a standard curve of protein concentration versus the proportion SIGNAL SIGNAL PROTEIN IN INTERNAL STANDARD. A PROTEIN THAT TYPICAL be measured by the method is ALBUMINA. Typically, measurements are made at 214nm and internal COMPOSITE STANDARD IS 2,4-dichlorobenzoic acid.